Introduction: problematic Smartphone Use (PSU) is a behavioral pattern characterized by conflicts in social relationships, avoidance of problems, lack of tolerance, and continued use of the device despite adverse consequences. The aim of this study is to identify the neurobiological mechanisms of PSU in adolescents and its impacts on mental health. Method: a systematic review was conducted according to the parameters of the PRISMA 2020 guidelines. Articles in English were searched in the SCOPUS, EBSCO, Web of Science, PubMed, and Medline databases, published between 2012 and 2022, and the risk of bias the identified studies were assessed using the Cochrane Handbook 5.1.0. Results: of the 314 articles initially identified, seven met the inclusion criteria. The neurobiological mechanism of PSU in adolescents is characterized by alterations in structures, functional connections, activities, and the concentration of certain biomolecules related to functions of the nervous system. Discussion: these changes lead to impacts on their mental health, such as issues with depression, behavior, impulsivity, and self-control.