Breast cancer remains a critical global health issue, necessitating continuous research and innovative approaches for diagnosis, treatment, and prevention. This study evaluates the effectiveness of U -Net models in enhancing diagnostic precision and efficiency using real hospital samples. We aim to improve key diagnostic metrics such as accuracy, sensitivity, and specificity through the application of U-Net models. Our image classification model, tailored for 256 × 256 × 3 input images, excels in detecting and categorizing tumor cells. The architecture begins with initial convolutional layers featuring 64 filters, progresses to layers with 128 filters, and includes a Dropout layer to prevent overfitting. The deep network for object detection utilizes both region proposal and regression/classification approaches, achieving 92.27% confidence and 100% accuracy. Additionally, our deep learning algorithms accurately segment nuclei in histopathological images, employing a clustering strategy that delivers 88.81% confidence and 100% accuracy. Visual results demonstrate precise tumor cell localization and prediction confidence. Performance metrics from ten experimental runs indicate average confidence levels between 74.19% and 92.31%, with 90.0% accuracy and specificity in benign analysis. The model's ability to classify non-carcinomas versus carcinomas achieved an AUC of 0.78, illustrating its effective differentiation between classes